Reporter

Part:BBa_K1311007:Design

Designed by: Luning Yang   Group: iGEM14_Tsinghua-A   (2014-10-09)

A reporter for the TALE protein binding ability


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5
    Illegal NheI site found at 28
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2131
    Illegal AgeI site found at 2243
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Promoter(J23102)-TALE binding site(repeats three times)-RBS-LacI coding sequence-Terminator(B0015)-LacI Regulative Promoter(R0010)-RBS(B0034)-mRFP1(E1010)-Terminator(B0015)

Our gene design on standard backbone.
Reportsystem3tsinghuaa.jpg


Our logic circuits.
%E6%9C%AA%E6%A0%87%E9%A2%98-1tsinghuaa.jpg

Source

It comes from other parts and a short oligonucleotide sequence as the TALE binding site.

References